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rt pcr mastercycler realplex machine  (Eppendorf AG)


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    Structured Review

    Eppendorf AG rt pcr mastercycler realplex machine
    Rt Pcr Mastercycler Realplex Machine, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 205264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+pcr+mastercycler+realplex+machine/Eppendorf/pm41037987-105-20-25
    Average 99 stars, based on 205264 article reviews
    rt pcr mastercycler realplex machine - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Tumor-Stroma Interactions Alter the Sensitivity of Drug in Breast Cancer
    Article Snippet: .. RT-PCR was performed using PerfeCTA R© SYBR Green FastMix (Quanta BioSciences, USA), following manufacturer’s instructions, on RT-PCR Mastercycler Realplex machine (Realplex, Eppendorf, Germany). .. Primer sequences (Eurofins Genomics, UK) were designed using Primer-BLAST tool (Table 1).

    Article Title: Bioinspired 3D Bone Model: Mimicking the Cortical-Spongy Bone Architecture and Biology for Enhanced Physiological Representation of Bone.
    Article Snippet: 3D bone models are essential for studying bone physiology, developing therapies, and reducing animal use.. Inspired by the hierarchical anatomy of the bone, we shaped previously developed gellan gum−hydroxyapatite spongy-like hydrogels into an outer ring and an inner disc to anatomically represent the cortical and spongy bone, respectively.. Biomaterial properties remained stable postmolding, with the polymeric networks absorbing up to 500% of water and exhibiting 4 kPa stiffness.

    Article Title: TNF-α driven inflammation in bone tissues using an anatomical- and bio-integrated hydroxyapatite-enriched spongy-like hydrogel 3D model.
    Article Snippet: Inflammatory bone diseases like osteoporosis affect over 200 million people globally, yet the mechanisms by which acute inflammation progresses to chronic remain poorly understood.. To address this, we developed an in vitro 3D cortical-sponge bone model of acute inflammation by introducing TNF-α.. The model consists of GG-HAp spongy-like hydrogels with osteoblasts from human mesenchymal stem cells (HBM-MSCs) in an outer compartment, and human dermal microvascular endothelial cells plus supporting HBM-MSCs in an inner compartment, mimicking cortical and trabecular bone, respectively.

    Article Title: adipoSIGHT in Therapeutic Response: Consequences in Osteosarcoma Treatment
    Article Snippet: .. RT-PCR was performed using PerfeCTA ® SYBR Green FastMix (Quanta BioSciences, Gaithersburg, MD, USA), following the manufacturer’s instructions, on the RT-PCR Mastercycler Realplex machine (Realplex, Eppendorf, Germany). .. Primer sequences (Eurofins Genomics, 23 Val Fleuri, Luxembourg) were designed using the Primer-BLAST tool ( ).

    Article Title: Bioinspired 3D Bone Model: Mimicking the Cortical–Spongy Bone Architecture and Biology for Enhanced Physiological Representation of Bone
    Article Snippet: This process was carried out using the qScript cDNA SuperMix synthesis kit (VWR, Leuven, Belgium) and the Eppendorf Mastercycler ep realplex gradient. .. The expression of bone-related genes listed in Supplementary Table S1 was assessed by an RT-PCR Mastercycler Realplex machine (Realplex, Eppendorf, Germany). .. To that, a PerfeCTAR©SYBR Green FastMix kit (Quanta BioSciences) was used according to the manufacturer’s instructions with the reaction conditions consisting of an initial incubation at 95 °C for 2 min, followed by 45 cycles of denaturation (95 °C, 10 s), annealing (specific to each gene, 25 s), and extension (72 °C, 30 s).

    Article Title: Mechanical Property of Hydrogels and the Presence of Adipose Stem Cells in Tumor Stroma Affect Spheroid Formation in the 3D Osteosarcoma Model.
    Article Snippet: RT-PCR was performed using Perfecta SYBR Green FastMix (Quanta BioSciences), following manufacturer’s instructions, on the RT-PCR Mastercycler realplex machine (Realplex, Eppendorf).

    SYBR Green Assay:

    Article Title: Tumor-Stroma Interactions Alter the Sensitivity of Drug in Breast Cancer
    Article Snippet: .. RT-PCR was performed using PerfeCTA R© SYBR Green FastMix (Quanta BioSciences, USA), following manufacturer’s instructions, on RT-PCR Mastercycler Realplex machine (Realplex, Eppendorf, Germany). .. Primer sequences (Eurofins Genomics, UK) were designed using Primer-BLAST tool (Table 1).

    Article Title: adipoSIGHT in Therapeutic Response: Consequences in Osteosarcoma Treatment
    Article Snippet: .. RT-PCR was performed using PerfeCTA ® SYBR Green FastMix (Quanta BioSciences, Gaithersburg, MD, USA), following the manufacturer’s instructions, on the RT-PCR Mastercycler Realplex machine (Realplex, Eppendorf, Germany). .. Primer sequences (Eurofins Genomics, 23 Val Fleuri, Luxembourg) were designed using the Primer-BLAST tool ( ).

    Article Title: Mechanical Property of Hydrogels and the Presence of Adipose Stem Cells in Tumor Stroma Affect Spheroid Formation in the 3D Osteosarcoma Model.
    Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Article Title: Mechanical Property of Hydrogels and the Presence of Adipose Stem Cells in Tumor Stroma Affect Spheroid Formation in the 3D Osteosarcoma Model.
    Article Snippet: RT-PCR was performed using Perfecta SYBR Green FastMix (Quanta BioSciences), following manufacturer’s instructions, on the RT-PCR Mastercycler realplex machine (Realplex, Eppendorf).

    Expressing:

    Article Title: Bioinspired 3D Bone Model: Mimicking the Cortical-Spongy Bone Architecture and Biology for Enhanced Physiological Representation of Bone.
    Article Snippet: 3D bone models are essential for studying bone physiology, developing therapies, and reducing animal use.. Inspired by the hierarchical anatomy of the bone, we shaped previously developed gellan gum−hydroxyapatite spongy-like hydrogels into an outer ring and an inner disc to anatomically represent the cortical and spongy bone, respectively.. Biomaterial properties remained stable postmolding, with the polymeric networks absorbing up to 500% of water and exhibiting 4 kPa stiffness.

    Article Title: Bioinspired 3D Bone Model: Mimicking the Cortical–Spongy Bone Architecture and Biology for Enhanced Physiological Representation of Bone
    Article Snippet: This process was carried out using the qScript cDNA SuperMix synthesis kit (VWR, Leuven, Belgium) and the Eppendorf Mastercycler ep realplex gradient. .. The expression of bone-related genes listed in Supplementary Table S1 was assessed by an RT-PCR Mastercycler Realplex machine (Realplex, Eppendorf, Germany). .. To that, a PerfeCTAR©SYBR Green FastMix kit (Quanta BioSciences) was used according to the manufacturer’s instructions with the reaction conditions consisting of an initial incubation at 95 °C for 2 min, followed by 45 cycles of denaturation (95 °C, 10 s), annealing (specific to each gene, 25 s), and extension (72 °C, 30 s).

    Gene Expression:

    Article Title: TNF-α driven inflammation in bone tissues using an anatomical- and bio-integrated hydroxyapatite-enriched spongy-like hydrogel 3D model.
    Article Snippet: Inflammatory bone diseases like osteoporosis affect over 200 million people globally, yet the mechanisms by which acute inflammation progresses to chronic remain poorly understood.. To address this, we developed an in vitro 3D cortical-sponge bone model of acute inflammation by introducing TNF-α.. The model consists of GG-HAp spongy-like hydrogels with osteoblasts from human mesenchymal stem cells (HBM-MSCs) in an outer compartment, and human dermal microvascular endothelial cells plus supporting HBM-MSCs in an inner compartment, mimicking cortical and trabecular bone, respectively.



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